2. Separating a redish colour from the black CD-R PEN
y In the previous experiment a redish material could be seen around some of the numbers written on the fabrics by a black CD-R Pen.
In another experiment the black pen Stabilo point 88 proved to contain a mixture. It was separated by paper chromatography.
You will use the same method to investigate the black CD-R Pen.
y .x .x .c
Materials
Tray
, ampoule 5 mL, stopper of an infusion bottle, black TDK CD-R PEN, pencil lead, filter paper  circle 5.5 cm Whatman, water,  toilet paper.

Experiment
1. Use a pencil lead to draw a circle in the middle of the filter paper  and then trace it with the CD-R PEN (Fig. 1).
2. Make a hole in the middle of the paper.
3. Twist a wick with a half piece of toilet paper, of water  and placed in a stopper as stand (Fig. 2).
5. Wait until the water moved up in the wick, reached the  black circle (Fig. 3) and finally the rim of the paper circle (Fig. 4).
Observations
1. Water moves upwards in the wick and horizontally in the filter paper.
2. A reddish substance leaves the black ring, while the front of the water passed through the filter paper.
Explanation
1. The cellulose fibres of filter paper leave space between each other like the fibres in the fabrics. Capillary forces move the water in the
     papers of the wick and the filter.

2. The separation of the reddish substance is due to its limited solubility in cold water, the movement of the water by capillarity and the limited
     adsorption to the paper.   In this process called paper chromatography the cellulose fibres are the stationary phase and the water is the
     mobile phase.



back.........................first published: 22.02.2006......................           last modification: 08.03.2007